ENZYME-SPECIFIC PROFILES for metabolic pathway prediction

PRIAM: PRofils pour l'Identification Automatique du Métabolisme


The prediction of metabolic pathways of many species is becoming possible with the advent of complete sequence genomes, on the basis of the identification of enzyme coding genes.
PRIAM is a method for automated enzyme detection in a fully sequenced genome, based on all sequences available in the ENZYME database.
PRIAM relies on sets of position-specific score matrices (PSSMs) automatically tailored for each ENZYME entry. The whole Swiss-Prot database has been used to parameterise and to assess the method.
As an example, PRIAM was applied to predict metabolic pathways from the complete genome of the nitrogen fixing bacterium Sinorhizobium meliloti and on the plant pathogen Ralstonia solanacearum. The results of this automated annotation method were compared with the original genome annotation and visualised on KEGG graphs in order to facilitate the interpretation of predicted metabolic pathways and to highlight potentially missing enzymes.

Release of October 2001 (Enzyme rel. 27.0)

Information on profiles of PRIAM

by EC number:
  • ( EC for example: )

  • Search for a protein

    RPSBLAST with PRIAM (oct 2001)
  • ( Choose for database: profil_ENZYME_27 )

  • Complete proteome analysis package

    Install PRIAM


    On Sinorhizobium meliloti:

    On Ralstonia solanacearum:

    For information, comments and / or suggestions on PRIAM,
    please email us at PRIAM Team